Article
Association and expression analysis of single nucleotide polymorphisms of partial tumor necrosis factor alpha gene with mastitis in crossbred cattle.
Animal biotechnology - 1 Jan 2015
Ranjan Sanjeev, Bhushan Bharat, Panigrahi Manjit, Kumar Amit, Deb Rajib, Kumar Pushpendra, Sharma Deepak
Abstract excerpt
A total of 129 crossbred cows were selected to explore the genotypic and expression profiling of partial TNF-α gene and its association with mastitis susceptibility. Two exon spanning region of TNF-α gene (221 bp and 239 bp) were amplified by Polymerase Chain Reaction (PCR). The different genotypic analysis by SSCP revealed that 221 bp fragment was monomorphic, whereas 239 bp was polymorphic. Association studies...
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