Article
SNP genotyping using KASPar assays.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015
Smith Scott M, Maughan Peter J
Abstract excerpt
In a separate chapter we describe a simple method for single nucleotide polymorphism (SNP) discovery using genomic reduction. Here we describe a scalable and cost-effective SNP genotyping method based on KBioscience's competitive allele-specific PCR amplification of target sequences and endpoint fluorescence genotyping (KASPar™) using a FRET capable plate reader or Fluidigm's dynamic array high-throughput platform.
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