Article
Novel approach to identifying the hepatitis B virus pre-S deletions associated with hepatocellular carcinoma.
World journal of gastroenterology - 7 Oct 2014
Zhao Zhi-Mei, Jin Yan, Gan Yu, Zhu Yu, Chen Tao-Yang, Wang Jin-Bing, Sun Yan, Cao Zhi-Gang, Qian Geng-Sun, Tu Hong
Abstract excerpt
AIM: To develop a novel non-sequencing method for the detection of hepatitis B virus (HBV) pre-S deletion mutants in HBV carriers. METHODS: The entire region of HBV pre-S1 and pre-S2 was amplified by polymerase chain reaction (PCR). The size of PCR products was subsequently determined by capillary gel electrophoresis (CGE). CGE were carried out in a PACE-MDQ instrument equipped with a UV detector set at 254 nm....
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