Article
Real-time optical studies of respiratory Complex I turnover.
Biochimica et biophysica acta - 1 Dec 2014
Belevich Nikolai, Belevich Galina, Verkhovskaya Marina
Abstract excerpt
Reduction of Complex l (NADH:ubiquinone oxidoreductase l) from Escherichia coli by NADH was investigated optically by means of an ultrafast stopped-flow approach. A locally designed microfluidic stopped-flow apparatus with a low volume (0.21Jl) but a long optical path (10 mm) cuvette allowed measurements in the time range from 270 ).IS to seconds. The data acquisition system collected spectra in the visible range...
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