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Fluorescence detection of KRAS2 mRNA hybridization in lung cancer cells with PNA-peptides containing an internal thiazole orange.

Bioconjugate chemistry - 17 Sept 2014

Sonar Mahesh V, Wampole Matthew E, Jin Yuan-Yuan, Chen Chang-Po, Thakur Mathew L, Wickstrom Eric

Abstract excerpt

We previously developed reporter-peptide nucleic acid (PNA)-peptides for sequence-specific radioimaging and fluorescence imaging of particular mRNAs in cells and tumors. However, a direct test for PNA-peptide hybridization with RNA in the cytoplasm would be desirable. Thiazole orange (TO) dye at the 5' end of a hybridization agent shows a strong increase in fluorescence quantum yield when stacked upon a 5'...

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Identifiers

PMID: 25180641
DOI: 10.1021/bc500304m
PMCID: PMC4166030

Genes

IGF1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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