Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A Reliable and Efficient Protocol for Human Pluripotent Stem Cell Differentiation into the Definitive Endoderm Based on Dispersed Single Cells

19 Aug 2014

Abstract excerpt

Differentiation of pluripotent cells into endoderm-related cell types initially requires in vitro gastrulation into the definitive endoderm (DE). Most differentiation protocols are initiated from colonies of pluripotent cells complicating their adaption due to insufficiently defined starting conditions. The protocol described here was initiated from a defined cell number of dispersed single cells and tested on...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CRISPR and Genetic Engineering
Share to Pancreatic function and diabetes
Share to Pluripotent Stem Cells Research

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 25137387
DOI: 10.1089/scd.2014.0143

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A Reliable and Efficient Protocol for Human Pluripotent Stem Cell Differentiation into the Definitive Endoderm Based on Dispersed Single CellsPMID 25137387Read ↗

A critical look: Challenges in differentiating human pluripotent stem cells into desired cell types and organoidsSemantic proximity Read ↗

Generation of Multipotent Foregut Stem Cells from Human Pluripotent Stem CellsSemantic proximity Read ↗

Towards consistent generation of pancreatic lineage progenitors from human pluripotent stem cellsSemantic proximity Read ↗

Stepwise differentiation of functional pancreatic β cells from human pluripotent stem cellsSemantic proximity Read ↗

Generation of pancreatic progenitors from human pluripotent stem cells by small moleculesSemantic proximity Read ↗

Comparable Generation of Activin-Induced Definitive Endoderm via Additive Wnt or BMP Signaling in Absence of SerumSemantic proximity Read ↗

Chemically defined and xenogeneic-free differentiation of human pluripotent stem cells into definitive endoderm in 3D cultureSemantic proximity Read ↗

Optimization of activin-A: a breakthrough in differentiation of human induced pluripotent stem cell into definitive endodermSemantic proximity Read ↗

Production and cryopreservation of definitive endoderm from human pluripotent stem cells under defined and scalable culture conditionsSemantic proximity Read ↗

Endogenous suppression of WNT signalling in human embryonic stem cells leads to low differentiation propensity towards definitive endodermSemantic proximity Read ↗

Endodermal differentiation of human pluripotent stem cells to insulin-producing cells in 3D culture.Semantic proximity Read ↗

Efficient definitive endoderm induction from mouse embryonic stem cell adherent cultures: A rapid screening model for differentiation studiesSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.