Article
Development of a multiplex PCR system of 59 mitochondrial SNPs and genetic analysis in Chinese population.
Electrophoresis - 1 Jul 2014
Nie Yanchai, Zhang Chen, Jiao Haitao, Zhao Ziqin, Zhou Huaigu
Abstract excerpt
The analysis of SNPs located on the mitochondrial DNA can provide information on maternal genetics. In the present study, a set of 59 SNPs were detected simultaneously using three multiplex allele-specific PCR and subsequent CE. Allele-specific primers were designed with different sizes to allow for specifically amplified paired alleles in the same reaction. An allelic ladder based on reference alleles was also...
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