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Article

Cell-autonomous-like silencing of GFP-partitioned transgenic Nicotiana benthamiana.

Journal of experimental botany - 1 Aug 2014

Sohn Seong-Han, Frost Jennifer, Kim Yoon-Hee, Choi Seung-Kook, Lee Yi, Seo Mi-Suk, Lim Sun-Hyung, Choi Yeonhee, Kim Kook-Hyung, Lomonossoff George

Abstract excerpt

We previously reported the novel partitioning of regional GFP-silencing on leaves of 35S-GFP transgenic plants, coining the term "partitioned silencing". We set out to delineate the mechanism of partitioned silencing. Here, we report that the partitioned plants were hemizygous for the transgene, possessing two direct-repeat copies of 35S-GFP. The detection of both siRNA expression (21 and 24 nt) and DNA...

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PMID: 24868037
DOI: 10.1093/jxb/eru200

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