Article
Trans-generation inheritance of methylation patterns in a tobacco transgene following a post-transcriptional silencing event.
The Plant journal : for cell and molecular biology - 1 Jun 2008
Lunerová-Bedrichová Jana, Bleys Annick, Fojtová Miloslava, Khaitová Lucie, Depicker Anna, Kovarík Ales
Abstract excerpt
We have studied the inheritance of the epigenetic state of tobacco transgenes whose expression was post-transcriptionally silenced by an invertedly repeated silencer locus. We show that, in hybrids, the coding region of the target neomycin phosphotransferase (nptII) gene was almost exclusively methylated at CG configurations, and dense non-CG methylation occurred in the 3' untranslated region. Homologous...
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