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Enzyme architecture: the effect of replacement and deletion mutations of loop 6 on catalysis by triosephosphate isomerase.

Biochemistry - 3 Jun 2014

Zhai Xiang, Go Maybelle K, O'Donoghue AnnMarie C, Amyes Tina L, Pegan Scott D, Wang Yan, Loria J Patrick, Mesecar Andrew D, Richard John P

Abstract excerpt

Two mutations of the phosphodianion gripper loop in chicken muscle triosephosphate isomerase (cTIM) were examined: (1) the loop deletion mutant (LDM) formed by removal of residues 170-173 [Pompliano, D. L., et al. (1990) Biochemistry 29, 3186-3194] and (2) the loop 6 replacement mutant (L6RM), in which the N-terminal hinge sequence of TIM from eukaryotes, 166-PXW-168 (X = L or V), is replaced by the sequence from...

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Identifiers

PMID: 24825099
DOI: 10.1021/bi500458t
PMCID: PMC4051426

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Source receipt: pubmed, retrieved 7 Sept 2026.

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