Article
Multiplex-Ready Technology for mid-throughput genotyping of molecular markers.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014
Bonneau Julien, Hayden Matthew
Abstract excerpt
Screening molecular markers across large populations in breeding programs is generally time consuming and expensive. The Multiplex-Ready Technology (MRT) (Hayden et al., BMC genomics 9:80, 2008) was created to optimize polymorphism screening and genotyping using standardized PCR reaction conditions. The flexibility of this method maximizes the number of markers (up to 24 markers SSR or SNP, ideally small PCR...
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