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Article

Single-nucleotide polymorphism PCR for the detection of Mycoplasma pneumoniae and determination of macrolide resistance in respiratory samples.

Journal of microbiological methods - 1 Jul 2014

Ji Misuk, Lee Nam-Sihk, Oh Ji-Min, Jo Ji Yoon, Choi Eun Hwa, Yoo Soo Jin, Kim Hyo-Bin, Hwang Sang-Hyun, Choi Sang-Ho, Lee Sang-Oh, Kim Mi-Na, Sung Heungsup

Abstract excerpt

The aim of this study was to develop a single-nucleotide polymorphism (SNP) PCR assay to be performed directly on respiratory samples for the simultaneous detection of Mycoplasma pneumoniae and its 23S rRNA gene mutations, which are responsible for macrolide resistance. For multiplex SNP PCR, two outer primers for amplification of the 23S rRNA gene and two mutant-specific primers for the discrimination of single...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 24780151
DOI: 10.1016/j.mimet.2014.04.009

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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