Multiplex amplicon sequencing for the comprehensive genotyping of Mycoplasma pneumoniae

Abstract excerpt

ABSTRACT Major genotyping methods used to characterize Mycoplasma pneumoniae strains are based on various experimental approaches that need to be implemented in parallel for each strain. In this study, we developed a comprehensive workflow based on amplicon sequencing using next-generation sequencing. This workflow comprised PCR amplification with seven tubes, collection into single tubes, shotgun sequencing, de...

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