Article
Mutational analysis of a plant branchpoint and polypyrimidine tract required for constitutive splicing of a mini-exon.
RNA (New York, N.Y.) - 1 Jan 2002
Simpson Craig G, Thow Graham, Clark Gillian P, Jennings S Nikki, Watters Jenny A, Brown John W S
Abstract excerpt
The branchpoint sequence and associated polypyrimidine tract are firmly established splicing signals in vertebrates. In plants, however, these signals have not been characterized in detail. The potato invertase mini-exon 2 (9 nt) requires a branchpoint sequence positioned around 50 nt upstream of the 5' splice site of the neighboring intron and a U11 element found adjacent to the branchpoint in the upstream...
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