Article
The pentatricopeptide repeat gene OTP43 is required for trans-splicing of the mitochondrial nad1 Intron 1 in Arabidopsis thaliana.
The Plant cell - 1 Oct 2007
de Longevialle Andéol Falcon, Meyer Etienne H, Andrés Charles, Taylor Nicolas L, Lurin Claire, Millar A Harvey, Small Ian D
Abstract excerpt
The mitochondrial NADH:ubiquinone oxidoreductase complex (Complex I) is a large protein complex formed from both nuclearly and mitochondrially encoded subunits. Subunit ND1 is encoded by a mitochondrial gene comprising five exons, and the mature transcript requires four RNA splicing events, two of which involve trans-splicing independently transcribed RNAs. We have identified a nuclear gene (OTP43) absolutely...
Topics
- Arabidopsis
- Arabidopsis Proteins
- Base Pairing
- DNA, Bacterial
- Electron Transport Complex I
- Electrophoresis, Polyacrylamide Gel
- Exons
- Gene Expression Regulation, Plant
- Genetic Complementation Test
- Immunoblotting