Typification of virulent and low virulence Babesia bigemina clones by 18S rRNA and rap-1c.

doi:10.1016/j.exppara.2014.03.016

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Article

Typification of virulent and low virulence Babesia bigemina clones by 18S rRNA and rap-1c.

Experimental parasitology - 1 Jun 2014

Thompson C, Baravalle M E, Valentini B, Mangold A, Torioni de Echaide S, Ruybal P, Farber M, Echaide I

Abstract excerpt

The population structure of original Babesia bigemina isolates and reference strains with a defined phenotypic profile was assessed using 18S rRNA and rap-1c genes. Two reference strains, BbiS2P-c (virulent) and BbiS1A-c (low virulence), were biologically cloned in vitro. The virulence profile of the strains and clones was assessed in vivo. One fully virulent and one low-virulence clone were mixed in identical...

Topics

Animals
Argentina
Babesia
Babesiosis
Base Sequence
Cattle
Cattle Diseases
Cloning, Molecular
DNA, Protozoan
Erythrocytes
Genotype
Genotyping Techniques
Haplotypes
Male
Molecular Sequence Data

Source

Open in PubMed

Identifiers

PMID: 24681200
DOI: 10.1016/j.exppara.2014.03.016

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protozoan Proteins

RNA, Ribosomal, 18S

Real-Time Polymerase Chain Reaction