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Article

Genotyping of the protozoan pathogen Toxoplasma gondii using high-resolution melting analysis of the repeated B1 gene.

Journal of microbiological methods - 1 Sept 2011

Costa Jean-Marc, Cabaret Odile, Moukoury Sandrine, Bretagne Stéphane

Abstract excerpt

Genetic studies of the protozoan parasite Toxoplasma gondii have identified three main distinct types according to virulence in some hosts. Several methods have been developed to differentiate genotypes currently dominated by microsatellite markers targeting single-copy loci. We analyzed the possibility of using the 35-fold repetitive B1 gene via high-resolution melting (HRM) curve analysis. Sequencing of the B1...

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PMID: 21741999
DOI: 10.1016/j.mimet.2011.06.017

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