Article
Developing a multiplex mtSNP assay for forensic application in Han Chinese based on mtDNA phylogeny and hot spot.
Electrophoresis - 1 Feb 2015
Ren Zheng, Luo Haibo, Song Feng, Wei Wei, Yang Yuyou, Zhai Xiandun, Chen Xiaogang, Hou Yiping
Abstract excerpt
We designed one multiplex assay with a reduced number of SNPs from whole mitochondrial genome as a screening approach for forensic purposes and developed a multiplex SNaPshot assay with 26 mitochondrial SNPs (mtSNPs). This assay included 16 target mtSNPs that defined the main haplogroups in Chinese population and ten hot-spot mtSNPs found by pyrosequencing. To validate our multiplex mtSNP assay, we not only...
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