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Ultrasensitive Norovirus Detection Using DNA Aptasensor Technology

14 Nov 2013

Abstract excerpt

DNA aptamers were developed against murine norovirus (MNV) using SELEX (Systematic Evolution of Ligands by EXponential enrichment). Nine rounds of SELEX led to the discovery of AG3, a promising aptamer with very high affinity for MNV as well as for lab-synthesized capsids of a common human norovirus (HuNoV) outbreak strain (GII.3). Using fluorescence anisotropy, AG3 was found to bind with MNV with affinity in the...

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PMID: 24244426
DOI: 10.1371/journal.pone.0079087

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