Article
Use of the Illumina GoldenGate assay for single nucleotide polymorphism (SNP) genotyping in cereal crops.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015
Chao Shiaoman, Lawley Cindy
Abstract excerpt
Highly parallel genotyping assays, such as the GoldenGate assay developed by Illumina, capable of interrogating up to 3,072 single nucleotide polymorphisms (SNPs) simultaneously, have greatly facilitated genome-wide studies, particularly for crops with large and complex genome structures. In this report, we provide detailed information and guidelines regarding genomic DNA preparation, SNP assay design, SNP assay...
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