Virus-induced gene silencing for comparative functional studies in Gladiolus hybridus.

doi:10.1007/s00299-013-1530-2

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Article

Virus-induced gene silencing for comparative functional studies in Gladiolus hybridus.

Plant cell reports - 1 Feb 2014

Zhong Xionghui, Yuan Xue, Wu Ze, Khan Muhammad Ali, Chen Jin, Li Xiaoxin, Gong Benhe, Zhao Yang, Wu Jian, Wu Chenyu, Yi Mingfang

Abstract excerpt

Functional analysis of genes in gladiolus has previously been impractical due to the lack of an efficient stable genetic transformation method. However, virus-induced gene silencing (VIGS) is effective in some plants which are difficult to transform through other methods. Although the Tobacco rattle virus (TRV)-based VIGS system has been developed and used for verifying gene functions in diverse plants, an...

Topics

Gene Expression Regulation, Plant
Gene Silencing
Genetic Vectors
Green Fluorescent Proteins
Iridaceae
Oxidoreductases
Phenotype
Plant Leaves
Plant Proteins
Plant Roots
Plants, Genetically Modified
Recombinant Fusion Proteins
Seedlings

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Sequence Analysis, DNA

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PMID: 24170343
DOI: 10.1007/s00299-013-1530-2

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