Article
TRV-GFP: a modified Tobacco rattle virus vector for efficient and visualizable analysis of gene function.
Journal of experimental botany - 1 Jan 2014
Tian Ji, Pei Haixia, Zhang Shuai, Chen Jiwei, Chen Wen, Yang Ruoyun, Meng Yonglu, You Jie, Gao Junping, Ma Nan
Abstract excerpt
Virus-induced gene silencing (VIGS) is a useful tool for functional characterization of genes in plants. Unfortunately, the efficiency of infection by Tobacco rattle virus (TRV) is relatively low for some non-Solanaceae plants, which are economically important, such as rose (Rosa sp.). Here, to generate an easy traceable TRV vector, a green fluorescent protein (GFP) gene was tagged to the 3' terminus of the coat...
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