Article
gadA gene locus in Lactobacillus brevis NCL912 and its expression during fed-batch fermentation.
FEMS microbiology letters - 1 Dec 2013
Li Haixing, Li Wenming, Liu Xiaohua, Cao Yusheng
Abstract excerpt
Normally, Lactobacillus brevis has two glutamate decarboxylase (GAD) genes; gadA and gadB. Using PCR, we cloned the gadA gene from L. brevis strain NCL912, a high yield strain for the production of gamma-aminobutyric acid (GABA). However, despite using 61 different primer pairs, including degenerate primers from conserved regions, we were unable to use PCR to clone gadB from the NCL912 strain. Furthermore, we...
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