Article
Key elements of the human bocavirus type 1 (HBoV1) promoter and its trans-activation by NS1 protein.
Virology journal - 27 Oct 2013
Li Jingjing, Yang Yongbo, Dong Yanming, Li Yongshu, Huang Yu, Yi Qianhui, Liu Kaiyu, Li Yi
Abstract excerpt
BACKGROUND: Human bocavirus (HBoV), a parvovirus, is suspected to be an etiologic agent of respiratory disease and gastrointestinal disease in humans. All mRNAs of HBoV1 are transcribed from a single promoter. METHODS: In this study, we constructed EGFP and luciferase reporter gene vectors under the control of the HBoV1 full promoter (nt 1-252) and its mutated variants, respectively. Fluorescence microscopy was...
Topics
- Artificial Gene Fusion
- Cell Line
- DNA Mutational Analysis
- Genes, Reporter
- Green Fluorescent Proteins
- Human bocavirus
- Humans
- Luciferases
- Microscopy, Fluorescence
- Mutation
- Promoter Regions, Genetic
- Trans-Activators
- Transcriptional Activation
- Viral Nonstructural Proteins