Article
Characterization of Sulfolobus solfataricus β-galactosidase mutant F441Y expressed in Pichia pastoris.
Journal of the science of food and agriculture - 1 May 2014
Sun Xiaojun, Duan Xuguo, Wu Dan, Chen Jian, Wu Jing
Abstract excerpt
BACKGROUND: β-Galactosidases are used to synthesize galactooligosaccharides (GOS). Since thermostable β-galactosidases have the advantages of high optimal temperature, thermostability as well as prevention of bacterial contamination in the production of GOS, they have received more attention in research. In this study a mutant of Sulfolobus solfataricus β-galactosidase, F441Y, was expressed in Pichia pastoris...
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