Article
Target site of Escherichia coli ribosomal protein S15 on its messenger RNA. Conformation and interaction with the protein.
Journal of molecular biology - 20 Jan 1990
Philippe C, Portier C, Mougel M, Grunberg-Manago M, Ebel J P, Ehresmann B, Ehresmann C
Abstract excerpt
The regulatory site of ribosomal protein S15 has been located in the 5' non-coding region of the messenger, overlapping with the ribosome loading site. The conformation of an in vitro synthesized mRNA fragment, covering the 105 nucleotides upstream from the initiation codon and the four first codons of protein S15, has been monitored using chemical probes and RNase V1. Our results show that the RNA is organized...
Topics
- Base Composition
- Base Sequence
- Escherichia coli
- Genes, Bacterial
- Genes, Regulator
- Models, Genetic
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation