Article
Role of conserved nucleotides in building the 16 S rRNA binding site for ribosomal protein S15.
Journal of molecular biology - 26 Jan 2001
Serganov A, Bénard L, Portier C, Ennifar E, Garber M, Ehresmann B, Ehresmann C
Abstract excerpt
Ribosomal protein S15 recognizes a highly conserved target on 16 S rRNA, which consists of two distinct binding regions. Here, we used extensive site-directed mutagenesis on a Escherichia coli 16 S rRNA fragment containing the S15 binding site, to investigate the role of conserved nucleotides in protein recognition and to evaluate the relative contribution of the two sites. The effect of mutations on S15...
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