Structural requirements for trans activation of human immunodeficiency virus type 1 long terminal repeat-directed gene expression by tat: importance of base pairing, loop sequence, and bulges in the tat-responsive sequence.

doi:10.1128/JVI.64.3.1402-1406.1990

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Article

Structural requirements for trans activation of human immunodeficiency virus type 1 long terminal repeat-directed gene expression by tat: importance of base pairing, loop sequence, and bulges in the tat-responsive sequence.

Journal of virology - 1 Mar 1990

Roy S, Parkin N T, Rosen C, Itovitch J, Sonenberg N

Abstract excerpt

In order to elucidate the molecular mechanisms of action of the tat-responsive sequence, mutational analysis of the tat-responsive sequence was carried out. The most critical region comprised nucleotides +18 to +44 and included the 3-nucleotide bulge at positions +23 to +25, the loop sequence, and an intact stem. In addition, base pairing up to nucleotide +52 was required for the full magnitude of the...

Topics

Base Composition
Base Sequence
Gene Expression Regulation, Viral
Gene Products, tat
Genes, Viral
HIV-1
HeLa Cells
Humans
Molecular Sequence Data
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Nucleic Acid Conformation

RNA, Messenger

Repetitive Sequences, Nucleic Acid

Restriction Mapping

Ribonucleases

Trans-Activators

Transcriptional Activation

Transfection

Source

Open in PubMed Open full text

Identifiers

PMID: 2406460
DOI: 10.1128/JVI.64.3.1402-1406.1990
PMCID: PMC249266

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2406460-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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