Article
Adapted J6/JFH1-based Hepatitis C virus recombinants with genotype-specific NS4A show similar efficacies against lead protease inhibitors, alpha interferon, and a putative NS4A inhibitor.
Antimicrobial agents and chemotherapy - 1 Dec 2013
Gottwein Judith M, Jensen Sanne B, Serre Stéphanie B N, Ghanem Lubna, Scheel Troels K H, Jensen Tanja B, Krarup Henrik, Uzcategui Nathalie, Mikkelsen Lotte S, Bukh Jens
Abstract excerpt
To facilitate studies of hepatitis C virus (HCV) NS4A, we aimed at developing J6/JFH1-based recombinants with genotype 1- to 7-specific NS4A proteins. We developed efficient culture systems expressing NS4A proteins of genotypes (isolates) 1a (H77 and TN), 1b (J4), 2a (J6), 4a (ED43), 5a (SA13), 6a (HK6a), and 7a (QC69), with peak infectivity titers of ∼3.5 to 4.5 log10 focus-forming units per ml. Except for...
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