Article
Genetic and biochemical evaluation of eucaryotic membrane protein topology: multiple transmembrane domains of Saccharomyces cerevisiae 3-hydroxy-3-methylglutaryl coenzyme A reductase.
Molecular and cellular biology - 1 Feb 1990
Sengstag C, Stirling C, Schekman R, Rine J
Abstract excerpt
Both 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase isozymes of the yeast Saccharomyces cerevisiae are predicted to contain seven membrane-spanning domains. Previous work had established the utility of the histidinol dehydrogenase protein domain, encoded by HIS4C, as a topologically se...
Topics
- Amino Acids
- Cell Membrane
- Chromosome Deletion
- Cloning, Molecular
- Escherichia coli
- Genes, Fungal
- Hydroxymethylglutaryl CoA Reductases
- Isoenzymes
- Membrane Proteins
- Mutation
- Phenotype
- Plasmids
- Protein Conformation
- Recombinant Fusion Proteins
- Restriction Mapping