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Article

Nitrate reduction and the isolation of Nit- mutants in Hansenula polymorpha.

Microbiology (Reading, England) - 1 Aug 1998

Pignocchi Cristina, Berardi Enrico, Cox Brian S

Abstract excerpt

Hansenula polymorpha (syn. Pichia angusta) is able to grow on nitrate as sole nitrogen source. Nitrate reductase (NR) assays, optimized in crude extracts from nitrate-grown cells, revealed that NR preferentially used NADPH, but also used NADH, as electron donor and required FAD for maximum activity. NR activity was present in nitrate-grown and nitrite-grown cells, and was absent in cells grown in ammonium,...

Topics

Chlorates
Culture Media
Drug Resistance, Microbial
Electron Transport
Enzyme Activation
Genes, Fungal
Mutation
Nitrate Reductase
Nitrate Reductases
Nitrates
Nitrogen
Nitrogen Fixation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Oxidation-Reduction

Pichia

Source

Identifiers

PMID: 9720055
DOI: 10.1099/00221287-144-8-2323

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-9720055-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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