Article
Nitrate reduction and the isolation of Nit- mutants in Hansenula polymorpha.
Microbiology (Reading, England) - 1 Aug 1998
Pignocchi Cristina, Berardi Enrico, Cox Brian S
Abstract excerpt
Hansenula polymorpha (syn. Pichia angusta) is able to grow on nitrate as sole nitrogen source. Nitrate reductase (NR) assays, optimized in crude extracts from nitrate-grown cells, revealed that NR preferentially used NADPH, but also used NADH, as electron donor and required FAD for maximum activity. NR activity was present in nitrate-grown and nitrite-grown cells, and was absent in cells grown in ammonium,...
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