Article
Screening for host proteins with pro- and antiviral activity using high-throughput RNAi.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013
Griffiths Samantha J
Abstract excerpt
RNA interference (RNAi) describes the mechanism of posttranscriptional gene silencing by small (typically 18-24 nucleotides) RNA molecules and includes small-interfering RNAs (siRNAs) and microRNAs (miRNAs). As siRNAs and miRNAs are simple to use experimentally, they are easily adaptable to high-throughput methodologies and provide an ideal tool for genome-wide gene depletion studies. Over recent years RNAi has...
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