Article
IMGT/HighV QUEST paradigm for T cell receptor IMGT clonotype diversity and next generation repertoire immunoprofiling.
Nature communications - 1 Jan 2013
Li Shuo, Lefranc Marie-Paule, Miles John J, Alamyar Eltaf, Giudicelli Véronique, Duroux Patrice, Freeman J Douglas, Corbin Vincent D A, Scheerlinck Jean-Pierre, Frohman Michael A, Cameron Paul U, Plebanski Magdalena, Loveland Bruce, Burrows Scott R, Papenfuss Anthony T, Gowans Eric J
Abstract excerpt
T cell repertoire diversity and clonotype follow-up in vaccination, cancer, infectious and immune diseases represent a major challenge owing to the enormous complexity of the data generated. Here we describe a next generation methodology, which combines 5'RACE PCR, 454 sequencing and, for analysi...
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