Genetic instability in Streptomyces niveus plasmid pSN2: in vivo formation of deletion derivatives.

doi:10.1007/BF00245235

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Article

Genetic instability in Streptomyces niveus plasmid pSN2: in vivo formation of deletion derivatives.

Archives of microbiology - 1 Jan 1990

Hussain H A, Mitchell J I, Ritchie D A

Abstract excerpt

A plasmid designated pSN2 (molecular size 32.0 kb) was isolated from the wild type of Streptomyces niveus ATCC 19793. To permit phenotypic identification of pSN2 the 1.9 kb BclI fragment was replaced in vitro by the 1.1 kb BclI fragment of pIJ702 carrying the thiostrepton resistance (tsr) gene to...

Topics

Chromosome Deletion
Cloning, Molecular
DNA, Bacterial
Drug Resistance, Microbial
Electrophoresis, Agar Gel
Nucleic Acid Hybridization
Phenotype
Plasmids
Protoplasts
Restriction Mapping
Streptomyces
Thiostrepton
Transformation, Bacterial

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