Article
Genetic instability in Streptomyces niveus plasmid pSN2: in vivo formation of deletion derivatives.
Archives of microbiology - 1 Jan 1990
Hussain H A, Mitchell J I, Ritchie D A
Abstract excerpt
A plasmid designated pSN2 (molecular size 32.0 kb) was isolated from the wild type of Streptomyces niveus ATCC 19793. To permit phenotypic identification of pSN2 the 1.9 kb BclI fragment was replaced in vitro by the 1.1 kb BclI fragment of pIJ702 carrying the thiostrepton resistance (tsr) gene to form the plasmid pSN3. pSN3 transforms S. lividans to thiostrepton resistance at high frequency and is stably...
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