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Article

Neurospheres and glial cell cultures: immunocytochemistry for cell phenotyping.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013

Struckhoff Amanda Parker, Del Valle Luis

Abstract excerpt

Cell cultures constitute an important tool for research as a way to reproduce pathological processes in a controlled system. However, the culture of brain-derived cells in monolayer presents significant challenges that obscure the fidelity of in vitro results. After a few number of passages, glial and neuronal cells begin to lose their morphological characteristics, and most importantly, their specific cellular...

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PMID: 23975825
DOI: 10.1007/978-1-62703-640-5_10

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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