Article
Neurospheres and Glial Cell Cultures; from Plating to Cell Phenotyping.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021
Parker Struckhoff Amanda, Del Valle Luis
Abstract excerpt
Cell cultures constitute an important tool for research as a way to reproduce pathological processes in a controlled system. However, the culture of brain-derived cells in monolayer presents significant challenges that obscure the fidelity of in vitro results. This is because after a few number of passages, glial and neuronal cells begin to lose their morphological characteristics, and most importantly, their...
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