Article
A conditional lethal mutation in Rana grylio virus ORF 53R resulted in a marked reduction in virion formation.
Virus research - 6 Nov 2013
He Li-Bo, Gao Xiao-Chan, Ke Fei, Zhang Qi-Ya
Abstract excerpt
Rana grylio virus (RGV) is a pathogenic iridovirus that has resulted in high mortality in cultured frog. Here, a recombinant RGV (i53R-RGV-lacIO) containing the inducible lac repressor/operator system was constructed. i53R-RGV-lacIO was a conditional lethal mutant in which the expression of envelope protein 53R was regulated by IPTG. i53R-RGV-lacIO shared characteristics similar to RGV in the presence of IPTG....
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