Article
Mitochondrial DNA ligase is dispensable for the viability of cultured cells but essential for mtDNA maintenance.
The Journal of biological chemistry - 13 Sept 2013
Shokolenko Inna N, Fayzulin Rafik Z, Katyal Sachin, McKinnon Peter J, Wilson Glenn L, Alexeyev Mikhail F
Abstract excerpt
Multiple lines of evidence support the notion that DNA ligase III (LIG3), the only DNA ligase found in mitochondria, is essential for viability in both whole organisms and in cultured cells. Previous attempts to generate cells devoid of mitochondrial DNA ligase failed. Here, we report, for the first time, the derivation of viable LIG3-deficient mouse embryonic fibroblasts. These cells lack mtDNA and are...
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