Article
A rapid and sensitive method for measuring N-acetylglucosaminidase activity in cultured cells.
PloS one - 1 Jan 2013
Mauri Victor, Lotfi Parisa, Segatori Laura, Sardiello Marco
Abstract excerpt
A rapid and sensitive method to quantitatively assess N-acetylglucosaminidase (NAG) activity in cultured cells is highly desirable for both basic research and clinical studies. NAG activity is deficient in cells from patients with Mucopolysaccharidosis type IIIB (MPS IIIB) due to mutations in NAGLU, the gene that encodes NAG. Currently available techniques for measuring NAG activity in patient-derived cell lines...
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