In situ assessment of beta-hexosaminidase activity.

Article

In situ assessment of beta-hexosaminidase activity.

BioTechniques - 1 Sept 1995

Lacorazza H D, Jendoubi M

Abstract excerpt

We have adapted two methods to evaluate the beta-hexosaminidase (HEX) enzymatic activity in cultured cells, based on the use of (i) the fluorogenic substrate 4-methylumbelliferyl-6-sulfo-2-acetamido-2- deoxy-beta-D-glucopyranoside (MU-GlcNAc-6-SO4) and (ii) the naphthol AS-BI-N-acetyl-beta-D-gluc...

Topics

Acetylglucosamine
Cells, Cultured
Fibroblasts
Glucuronates
Histocytochemistry
Humans
Hymecromone
Isoenzymes
Mutation
Naphthols
Sandhoff Disease
Spectrometry, Fluorescence
Tay-Sachs Disease
beta-N-Acetylhexosaminidases

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