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Article

Improvement of LATE-PCR to allow single-cell analysis by pyrosequencing.

The Analyst - 7 Sept 2013

Song Qinxin, Yang Huiyong, Zou Bingjie, Kajiyama Tomoharu, Kambara Hideki, Zhou Guohua

Abstract excerpt

Nucleic acid analysis in a single cell is very important, but the extremely small amount of template in a single cell requires a detection method more sensitive than the conventional method. In this paper, we describe a novel assay allowing a single cell genotyping by coupling improved linear-after-the-exponential-PCR (imLATE-PCR) on a modified glass slide with highly sensitive pyrosequencing. Due to the...

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Identifiers

PMID: 23817386
DOI: 10.1039/c3an00821e

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BRCA1
PGD

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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