A procedure for enrichment and isolation of mutants of the salt-tolerant yeast Debaryomyces hansenii having altered glycerol metabolism.

doi:10.1016/0378-1097(90)90415-m

Back to search

Article

A procedure for enrichment and isolation of mutants of the salt-tolerant yeast Debaryomyces hansenii having altered glycerol metabolism.

FEMS microbiology letters - 1 May 1990

Morales C, André L, Adler L

Abstract excerpt

The salt-tolerant yeast Debaryomyces hansenii produces and accumulates glycerol when subjected to salt stress, whereby the buoyant density of the cells is changed. This property allows for enrichment of mutants with altered glycerol metabolism by density gradient centrifugation. Colonies derived...

Topics

Centrifugation, Density Gradient
Chromosome Mapping
Chromosomes, Fungal
Cloning, Molecular
DNA, Fungal
Electrophoresis, Agar Gel
Genes, Fungal
Glycerol
Karyotyping
Mutation
Saccharomycetales
Sodium Chloride

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.