Article
BRAFV600E immunohistochemistry facilitates universal screening of colorectal cancers for Lynch syndrome.
The American journal of surgical pathology - 1 Oct 2013
Toon Christopher W, Walsh Michael D, Chou Angela, Capper David, Clarkson Adele, Sioson Loretta, Clarke Stephen, Mead Scott, Walters Rhiannon J, Clendenning Mark, Rosty Christophe, Young Joanne P, Win Aung Ko, Hopper John L, Crook Ashley, von Deimling Andreas, Jenkins Mark A, Buchanan Daniel D, Gill Anthony J
Abstract excerpt
BRAFV600E mutation in microsatellite-unstable (MSI) colorectal carcinomas (CRCs) virtually excludes Lynch syndrome (LS). In microsatellite-stable (MSS) CRCs it predicts poor prognosis. We propose a universal CRC LS screening algorithm using concurrent reflex immunohistochemistry (IHC) for BRAFV600E and mismatch-repair (MMR) proteins. We compared BRAFV600E IHC with multiplex polymerase chain reaction (PCR) and...
Topics
- Algorithms
- Colorectal Neoplasms, Hereditary Nonpolyposis
- DNA Mismatch Repair
- Female
- Humans
- Immunohistochemistry
- Male
- Mass Screening
- Microsatellite Instability