Article
Fluorescent-based methods for gene knockdown and functional cardiac imaging in zebrafish.
Molecular biotechnology - 1 Oct 2013
Umemoto Noriko, Nishimura Yuhei, Shimada Yasuhito, Yamanaka Yukiko, Kishi Seiya, Ito Saki, Okamori Kana, Nakamura Yuuki, Kuroyanagi Junya, Zhang Zi, Zang Liqing, Wang Zhipeng, Nishimura Norihiro, Tanaka Toshio
Abstract excerpt
A notable advantage of zebrafish as a model organism is the ease of gene knockdown using morpholino antisense oligonucleotide (MO). However, zebrafish morphants injected with MO for a target protein often show heterogeneous phenotypes, despite controlling the injection volume of the MO solution i...
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