Article
Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats.
PloS one - 1 Jan 2014
Dealtry Simone, Ding Guo-Chun, Weichelt Viola, Dunon Vincent, Schlüter Andreas, Martini María Carla, Del Papa María Florencia, Lagares Antonio, Amos Gregory Charles Auton, Wellington Elizabeth Margaret Helen, Gaze William Hugo, Sipkema Detmer, Sjöling Sara, Springael Dirk, Heuer Holger, van Elsas Jan Dirk, Thomas Christopher, Smalla Kornelia
Abstract excerpt
IncP-1, IncP-7 and IncP-9 plasmids often carry genes encoding enzymes involved in the degradation of man-made and natural contaminants, thus contributing to bacterial survival in polluted environments. However, the lack of suitable molecular tools often limits the detection of these plasmids in the environment. In this study, PCR followed by Southern blot hybridization detected the presence of plasmid-specific...
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