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Article

Genotyping of 25 leukemia-associated genes in a single work flow by next-generation sequencing technology with low amounts of input template DNA.

Clinical chemistry - 1 Aug 2013

Rinke Jenny, Schäfer Vivien, Schmidt Mathias, Ziermann Janine, Kohlmann Alexander, Hochhaus Andreas, Ernst Thomas

Abstract excerpt

BACKGROUND: We sought to establish a convenient, sensitive next-generation sequencing (NGS) method for genotyping the 26 most commonly mutated leukemia-associated genes in a single work flow and to optimize this method for low amounts of input template DNA. METHODS: We designed 184 PCR amplicons that cover all of the candidate genes. NGS was performed with genomic DNA (gDNA) from a cohort of 10 individuals with...

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Identifiers

PMID: 23592507
DOI: 10.1373/clinchem.2013.204099

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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