Article
A trio of human molecular genetics PCR assays.
Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology - 1 Jan 2000
Reinking Jeffrey L, Waldo Jennifer T, Dinsmore Jannett
Abstract excerpt
This laboratory exercise demonstrates three different analytical forms of the polymerase chain reaction (PCR) that allow students to genotype themselves at four different loci. Here, we present protocols to allow students to a) genotype a non-coding polymorphic Variable Number of Tandem Repeat (VNTR) locus on human chromosome 5 using conventional PCR, b) perform PCR - Restriction Fragment Polymorphism (PCR-RFLP)...
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