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Affordable hands-on DNA sequencing and genotyping: an exercise for teaching DNA analysis to undergraduates.

Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology - 1 Jan 2000

Shah Kushani, Thomas Shelby, Stein Arnold

Abstract excerpt

In this report, we describe a 5-week laboratory exercise for undergraduate biology and biochemistry students in which students learn to sequence DNA and to genotype their DNA for selected single nucleotide polymorphisms (SNPs). Students use miniaturized DNA sequencing gels that require approximately 8 min to run. The students perform G, A, T, C Sanger sequencing reactions. They prepare and run the gels, perform...

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PMID: 24019265
DOI: 10.1002/bmb.20732

Genes

ABCC11

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