Article
Molecular phenotyping of a trinucleotide repeat (D5S373) experimental conditions.
Journal of forensic sciences - 1 Jan 2003
Luis J R, Dios S, Carril J C, Herrera R, Caeiro B
Abstract excerpt
The aim of this study is to assess the utility of the STR D5S373 in human identification. PCR amplification and electrophoretic separation were optimized in order to achieve unambiguous phenotyping. We concluded that primer concentration and annealing temperature are the main factors affecting the specificity of PCR. In our population survey including three human major groups (Europe, Sub-Saharan Africa, and...
Topics
- Alleles
- DNA Fingerprinting
- Electrophoresis, Polyacrylamide Gel
- Gene Frequency
- Genetics, Population
- Genotype
- Humans
- Molecular Sequence Data
- Phenotype
- Polymerase Chain Reaction
- Racial Groups
- Sensitivity and Specificity
- Sequence Analysis, DNA
- Trinucleotide Repeats