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Article

Magnitude and origin of the enhanced basicity of the catalytic glutamate of triosephosphate isomerase.

Journal of the American Chemical Society - 24 Apr 2013

Malabanan M Merced, Nitsch-Velasquez Lucia, Amyes Tina L, Richard John P

Abstract excerpt

Glu-167 of triosephosphate isomerase from Trypanosoma brucei brucei (TbbTIM) acts as the base to deprotonate substrate to form an enediolate phosphate trianion intermediate. We report that there is a large ~6 pK unit increase in the basicity of the carboxylate side chain of Glu-167 upon binding of the inhibitor phosphoglycolate trianion (I(3-)), an analog of the enediolate phosphate intermediate, from pKEH ≈ 4...

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Identifiers

PMID: 23560625
DOI: 10.1021/ja401504w
PMCID: PMC3649849

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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